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PGK1 抗体 (AA 117-145)

Name: PGK1 抗体 (AA 117-145)
SKU: ABIN391135
Availability: OutOfStock

This anti-PGK1 antibody is a 兔多克隆 antibody detecting PGK1 in WB, IF, IHC (p) 和 FACS. Suitable for 人. This Primary Antibody has been cited in 8+ publications.

Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (ABIN391135 and ABIN2841256). (PGK1 抗体 (AA 117-145))

Immunohistochemical analysis of paraffin-embedded H. (PGK1 抗体 (AA 117-145))

IHC (p)

Confocal immunofluorescent analysis of PGK1 Antibody (Center) (ABIN391135 and ABIN2841256) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PGK1 抗体 (AA 117-145))

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PGK1 抗体 (AA 117-145))

IHC (p)

PGK1 Antibody (Center) (ABIN391135 and ABIN2841256) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (PGK1 抗体 (AA 117-145))

FACS

5 images

(8 references)

产品编号 ABIN391135

发货至: 中国

Quick Overview for PGK1 抗体 (AA 117-145) (ABIN391135)

抗原

See all PGK1 抗体

PGK1 (Phosphoglycerate Kinase 1 (PGK1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This PGK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

克隆位点

RB05463

Discover our top product PGK1 Primary Antibody

抗原表位
7

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3

3

2

2

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2

1

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1

1

1

1
AA 117-145

预测反应

Hs, Pr, M, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.

亚型

Ig Fraction
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 2-417) antibody
PGK1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) antibody
PGK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) (AA 117-145) antibody
PGK1 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) antibody
PGK1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) antibody
PGK1 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 1086CT10-2-1 unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) antibody
PGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) (AA 321-417) antibody
PGK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H4 unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) antibody
PGK1 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 6C1 unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) (AA 321-417) antibody
PGK1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Phosphoglycerate Kinase 1 (PGK1) (AA 102-151) antibody
PGK1 适用: 人, 小鼠, 大鼠, Cow, 绵羊, 犬, 豚鼠, 马, 兔, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Chen, Liu, Wu, Sheu: "Antrodia cinnamomea Inhibits Migration in Human Hepatocellular Carcinoma Cells: The Role of ERp57 and PGK-1." in: The American journal of Chinese medicine, Vol. 43, Issue 8, pp. 1671-96, (2016) (PubMed).

Ho, Morrison, Silva, Coomber: "The effect of 3-bromopyruvate on human colorectal cancer cells is dependent on glucose concentration but not hexokinase II expression." in: Bioscience reports, Vol. 36, Issue 1, pp. e00299, (2016) (PubMed).

Xu, Fang, Zhu, Dai, Nie, Chen, Qin, Wang, Wang, Qiao: "Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis." in: Biology of reproduction, Vol. 89, Issue 6, pp. 142, (2013) (PubMed).

Danshina, Geyer, Dai, Goulding, Willis, Kitto, McCarrey, Eddy, OBrien: "Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice." in: Biology of reproduction, Vol. 82, Issue 1, pp. 136-45, (2009) (PubMed).

Doran, OConnell, Gannon, Kavanagh, Ohlendieck: "Opposite pathobiochemical fate of pyruvate kinase and adenylate kinase in aged rat skeletal muscle as revealed by proteomic DIGE analysis." in: Proteomics, Vol. 8, Issue 2, pp. 364-77, (2008) (PubMed).

Kilic, Kasperczyk, Fulda, Debatin: "Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance." in: Oncogene, Vol. 26, Issue 14, pp. 2027-38, (2007) (PubMed).

OConnell, Gannon, Doran, Ohlendieck: "Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle." in: International journal of molecular medicine, Vol. 20, Issue 2, pp. 145-53, (2007) (PubMed).

West, Moore, Biskup, Bugayenko, Smith, Ross, Dawson, Dawson: "Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 46, pp. 16842-7, (2005) (PubMed).
抗原

PGK1 (Phosphoglycerate Kinase 1 (PGK1))

别名

PGK1

背景

Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

分子量

44615

基因ID

5230

NCBI登录号

NP_000282

UniProt

P00558

途径

Cellular Glucan Metabolic Process