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PDK4 抗体 (C-Term)

Name: PDK4 抗体 (C-Term)
SKU: ABIN391039
Availability: OutOfStock

PDK4 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB03041 unconjugated

Anti-PDK4 Antibody (C-term) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 μg per lane.

IHC (p)

All lanes : Anti-PDK4 Antibody (C-term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane.

Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody.

All lanes : Anti-PDK4 Antibody at 1:2000 dilution Lane 1: human heart lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 μg per lane.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (ABIN391039 and ABIN2841206) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

6 images

(24 references)

产品编号 ABIN391039

发货至: 中国

Datasheet as PDF

抗原 See all PDK4 抗体

PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))
抗原表位
15

14

8

8

7

6

5

5

5

5

2

1

1

1

1

1

1

1

1

1
AA 382-410, C-Term
适用
72

46

9

2

2

2

2

1

1

1

1
人, 小鼠
宿主
71

13
兔
Compatible Secondaries
克隆类型
75

11
多克隆
标记
44

9

6

6

5

5

1

1

1

1

1

1

1

1

1

1

1
This PDK4 antibody is un-conjugated
应用范围
76

50

28

13

13

9

7

3

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.

克隆位点

RB03041

亚型

Ig Fraction
anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 117-387) antibody
PDK4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410) antibody
PDK4 适用: 人, 小鼠 WB, ELISA, IHC, IF, mIHC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (C-Term) antibody
PDK4 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 102-411) antibody
PDK4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 83-111) antibody
PDK4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 1-411) antibody
PDK4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1A10 unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (N-Term) antibody
PDK4 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 兔, 犬, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 143-333) antibody
PDK4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 1-411) antibody
PDK4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 1-411) antibody
PDK4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:100. WB: 1:2000. WB: 1:2000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Cole, Abd Jamil, Heather, Murray, Sutton, Slingo, Sebag-Montefiore, Tan, Aksentijević, Gildea, Stuckey, Yeoh, Carr, Evans, Aasum, Schofield, Ratcliffe, Neubauer, Robbins, Clarke: "On the pivotal role of PPARα in adaptation of the heart to hypoxia and why fat in the diet increases hypoxic injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 8, pp. 2684-97, (2017) (PubMed).

Zhang, Wu, Choiniere, Yang, Huang, Bennett, Wang: "Arsenic silences hepatic PDK4 expression through activation of histone H3K9 methylatransferase G9a." in: Toxicology and applied pharmacology, Vol. 304, pp. 42-7, (2017) (PubMed).

Zhang, Zhang, Geng, Yi, Huo, Talmon, Kim, Wang, Wang: "Transforming Growth Factor β Mediates Drug Resistance by Regulating the Expression of Pyruvate Dehydrogenase Kinase 4 in Colorectal Cancer." in: The Journal of biological chemistry, Vol. 291, Issue 33, pp. 17405-16, (2017) (PubMed).

Li, Li, Hu, Lei, Xiong, Ji, Yin, Wei, Hu: "The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis." in: Oncogene, Vol. 36, Issue 7, pp. 989-998, (2017) (PubMed).

Jiang, de Castro Barbosa, Massart, Deshmukh, Löfgren, Duque-Guimaraes, Ozilgen, Osler, Chibalin, Zierath: "Diacylglycerol kinase-δ regulates AMPK signaling, lipid metabolism, and skeletal muscle energetics." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 1, pp. E51-60, (2016) (PubMed).

Mazar, Qi, Lee, Marchica, Govindarajan, Shelley, Li, Ray, Perera: "MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells." in: Molecular and cellular biology, Vol. 36, Issue 7, pp. 1090-108, (2016) (PubMed).

Walter, Thomalla, Bruhn, Fagan, Zehowski, Yee, Skildum: "Altered regulation of PDK4 expression promotes antiestrogen resistance in human breast cancer cells." in: SpringerPlus, Vol. 4, pp. 689, (2015) (PubMed).

Kamarajugadda, Stemboroski, Cai, Simpson, Nayak, Tan, Lu: "Glucose oxidation modulates anoikis and tumor metastasis." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1893-907, (2012) (PubMed).

Haldar, Jeyaraj, Anand, Zhu, Lu, Prosdocimo, Eapen, Kawanami, Okutsu, Brotto, Fujioka, Kerner, Rosca, McGuinness, Snow, Russell, Gerber, Bai, Yan, Nosek, Brotto, Hoppel, Jain: "Kruppel-like factor 15 regulates skeletal muscle lipid flux and exercise adaptation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 17, pp. 6739-44, (2012) (PubMed).

Williams, Wan, Frier, Bell, Field, Wright: "Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 302, Issue 1, pp. R49-58, (2011) (PubMed).

Long, Kostovski, Boon, Hjeltnes, Krook, Widegren: "Differential expression of metabolic genes essential for glucose and lipid metabolism in skeletal muscle from spinal cord injured subjects." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 110, Issue 5, pp. 1204-10, (2011) (PubMed).

Phillips, Aponte, Covian, Balaban: "Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexes." in: Biochemistry, Vol. 50, Issue 13, pp. 2515-29, (2011) (PubMed).

Dunford, Herbst, Jeoung, Gittings, Inglis, Vandenboom, LeBlanc, Harris, Peters: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, (2011) (PubMed).

Attia, Connnaughton, Boone, Wang, Elam, Ness, Cook, Park: "Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by thyroid hormone: role of the peroxisome proliferator-activated receptor gamma coactivator (PGC-1 alpha)." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2375-85, (2010) (PubMed).

Dateki, Kunitomo, Yoshioka, Yanai, Nakasono, Negishi: "Adaptive gene regulation of pyruvate dehydrogenase kinase isoenzyme 4 in hepatotoxic chemical-induced liver injury and its stimulatory potential for DNA repair and cell proliferation." in: Journal of receptor and signal transduction research, Vol. 31, Issue 1, pp. 85-95, (2010) (PubMed).

Zhao, Okuyama, Hashimoto, Tagawa, Jomori, Yang: "Bezafibrate induces myotoxicity in human rhabdomyosarcoma cells via peroxisome proliferator-activated receptor alpha signaling." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 24, Issue 1, pp. 154-9, (2010) (PubMed).

Basu, Oudit, Wang, Zhang, Ussher, Lopaschuk, Kassiri: "Type 1 diabetic cardiomyopathy in the Akita (Ins2WT/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 6, pp. H2096-108, (2009) (PubMed).

Hsieh, Das, Sambandam, Zhang, Nahlé: "Regulation of the PDK4 isozyme by the Rb-E2F1 complex." in: The Journal of biological chemistry, Vol. 283, Issue 41, pp. 27410-7, (2008) (PubMed).

Nahlé, Hsieh, Pietka, Coburn, Grimaldi, Zhang, Das, Abumrad: "CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14317-26, (2008) (PubMed).

Long, Glund, Garcia-Roves, Zierath: "Calcineurin regulates skeletal muscle metabolism via coordinated changes in gene expression." in: The Journal of biological chemistry, Vol. 282, Issue 3, pp. 1607-14, (2007) (PubMed).

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抗原

PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

别名

PDK4 (PDK4 产品)

别名

AV005916 antibody, pyruvate dehydrogenase kinase 4 antibody, pyruvate dehydrogenase kinase, isoenzyme 4 antibody, pdk4 antibody, PDK4 antibody, Pdk4 antibody

背景

PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

分子量

46469

基因ID

5166

NCBI登录号

NP_002603

UniProt

Q16654

途径

PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect