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PDK4 抗体 (C-Term)

Name: PDK4 抗体 (C-Term)
SKU: ABIN391039
Availability: OutOfStock

Cited in 24+ publications. This anti-PDK4 antibody is a 兔多克隆 antibody detecting PDK4 in WB, IF 和 IHC (p). Suitable for 人和小鼠.

Anti-PDK4 Antibody (C-term) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 μg per lane. (PDK4 抗体 (C-Term))

IHC (p)

All lanes : Anti-PDK4 Antibody (C-term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. (PDK4 抗体 (C-Term))

Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody. (PDK4 抗体 (C-Term))

All lanes : Anti-PDK4 Antibody at 1:2000 dilution Lane 1: human heart lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 μg per lane. (PDK4 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (ABIN391039 and ABIN2841206) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PDK4 抗体 (C-Term))

IHC (p)

6 images

(24 references)

产品编号 ABIN391039

发货至: 中国

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Quick Overview for PDK4 抗体 (C-Term) (ABIN391039)

抗原

See all PDK4 抗体

PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This PDK4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB03041

抗原表位
15

10

8

7

5

5

5

2

2

1

1

1

1

1

1

1

1

1

1
AA 382-410, C-Term

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.

亚型

Ig Fraction
anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 117-387) antibody
PDK4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410) antibody
PDK4 适用: 人, 小鼠 WB, IHC, ELISA, IF, mIHC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 102-411) antibody
PDK4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 83-111) antibody
PDK4 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 1-411) antibody
PDK4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1A10 unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (N-Term) antibody
PDK4 适用: 人, 小鼠, 大鼠, 马, Pig, 兔, Cow, 犬, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 143-333) antibody
PDK4 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 1-411) antibody
PDK4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (C-Term) antibody
PDK4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (N-Term) antibody
PDK4 适用: 人, 马, Pig, 兔, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:100. WB: 1:2000. WB: 1:2000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Cole, Abd Jamil, Heather, Murray, Sutton, Slingo, Sebag-Montefiore, Tan, Aksentijević, Gildea, Stuckey, Yeoh, Carr, Evans, Aasum, Schofield, Ratcliffe, Neubauer, Robbins, Clarke: "On the pivotal role of PPARα in adaptation of the heart to hypoxia and why fat in the diet increases hypoxic injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 8, pp. 2684-97, (2017) (PubMed).

Zhang, Wu, Choiniere, Yang, Huang, Bennett, Wang: "Arsenic silences hepatic PDK4 expression through activation of histone H3K9 methylatransferase G9a." in: Toxicology and applied pharmacology, Vol. 304, pp. 42-7, (2017) (PubMed).

Zhang, Zhang, Geng, Yi, Huo, Talmon, Kim, Wang, Wang: "Transforming Growth Factor β Mediates Drug Resistance by Regulating the Expression of Pyruvate Dehydrogenase Kinase 4 in Colorectal Cancer." in: The Journal of biological chemistry, Vol. 291, Issue 33, pp. 17405-16, (2017) (PubMed).

Li, Li, Hu, Lei, Xiong, Ji, Yin, Wei, Hu: "The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis." in: Oncogene, Vol. 36, Issue 7, pp. 989-998, (2017) (PubMed).

Jiang, de Castro Barbosa, Massart, Deshmukh, Löfgren, Duque-Guimaraes, Ozilgen, Osler, Chibalin, Zierath: "Diacylglycerol kinase-δ regulates AMPK signaling, lipid metabolism, and skeletal muscle energetics." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 1, pp. E51-60, (2016) (PubMed).

Mazar, Qi, Lee, Marchica, Govindarajan, Shelley, Li, Ray, Perera: "MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells." in: Molecular and cellular biology, Vol. 36, Issue 7, pp. 1090-108, (2016) (PubMed).

Walter, Thomalla, Bruhn, Fagan, Zehowski, Yee, Skildum: "Altered regulation of PDK4 expression promotes antiestrogen resistance in human breast cancer cells." in: SpringerPlus, Vol. 4, pp. 689, (2015) (PubMed).

Kamarajugadda, Stemboroski, Cai, Simpson, Nayak, Tan, Lu: "Glucose oxidation modulates anoikis and tumor metastasis." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1893-907, (2012) (PubMed).

Haldar, Jeyaraj, Anand, Zhu, Lu, Prosdocimo, Eapen, Kawanami, Okutsu, Brotto, Fujioka, Kerner, Rosca, McGuinness, Snow, Russell, Gerber, Bai, Yan, Nosek, Brotto, Hoppel, Jain: "Kruppel-like factor 15 regulates skeletal muscle lipid flux and exercise adaptation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 17, pp. 6739-44, (2012) (PubMed).

Williams, Wan, Frier, Bell, Field, Wright: "Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 302, Issue 1, pp. R49-58, (2011) (PubMed).

Long, Kostovski, Boon, Hjeltnes, Krook, Widegren: "Differential expression of metabolic genes essential for glucose and lipid metabolism in skeletal muscle from spinal cord injured subjects." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 110, Issue 5, pp. 1204-10, (2011) (PubMed).

Phillips, Aponte, Covian, Balaban: "Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexes." in: Biochemistry, Vol. 50, Issue 13, pp. 2515-29, (2011) (PubMed).

Dunford, Herbst, Jeoung, Gittings, Inglis, Vandenboom, LeBlanc, Harris, Peters: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, (2011) (PubMed).

Attia, Connnaughton, Boone, Wang, Elam, Ness, Cook, Park: "Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by thyroid hormone: role of the peroxisome proliferator-activated receptor gamma coactivator (PGC-1 alpha)." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2375-85, (2010) (PubMed).

Dateki, Kunitomo, Yoshioka, Yanai, Nakasono, Negishi: "Adaptive gene regulation of pyruvate dehydrogenase kinase isoenzyme 4 in hepatotoxic chemical-induced liver injury and its stimulatory potential for DNA repair and cell proliferation." in: Journal of receptor and signal transduction research, Vol. 31, Issue 1, pp. 85-95, (2010) (PubMed).

Zhao, Okuyama, Hashimoto, Tagawa, Jomori, Yang: "Bezafibrate induces myotoxicity in human rhabdomyosarcoma cells via peroxisome proliferator-activated receptor alpha signaling." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 24, Issue 1, pp. 154-9, (2010) (PubMed).

Basu, Oudit, Wang, Zhang, Ussher, Lopaschuk, Kassiri: "Type 1 diabetic cardiomyopathy in the Akita (Ins2WT/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 6, pp. H2096-108, (2009) (PubMed).

Hsieh, Das, Sambandam, Zhang, Nahlé: "Regulation of the PDK4 isozyme by the Rb-E2F1 complex." in: The Journal of biological chemistry, Vol. 283, Issue 41, pp. 27410-7, (2008) (PubMed).

Nahlé, Hsieh, Pietka, Coburn, Grimaldi, Zhang, Das, Abumrad: "CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14317-26, (2008) (PubMed).

Long, Glund, Garcia-Roves, Zierath: "Calcineurin regulates skeletal muscle metabolism via coordinated changes in gene expression." in: The Journal of biological chemistry, Vol. 282, Issue 3, pp. 1607-14, (2007) (PubMed).

show more references
抗原

PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

别名

PDK4

背景

PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

分子量

46469

基因ID

5166

NCBI登录号

NP_002603

UniProt

Q16654

途径

PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect