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HA-Tag 抗体 (FITC)

IF, FACS 宿主: 小鼠 Monoclonal 5E11D8 FITC
产品编号 ABIN387723
发货至: 中国
  • 抗原 See all HA-Tag products
    HA-Tag
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This HA-Tag antibody is conjugated to FITC
    应用范围
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    Immunofluorescence (IF), Flow Cytometry (FACS)
    品牌
    THE™
    序列
    YPYDVPDYA
    特异性
    THETM HA Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal HA tagged fusion proteins.
    产品特性
    This product is suitable for detecting the expression level of HA fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
    纯化方法
    Protein A affinity column
    免疫原
    A synthetic peptide YPYDVPDYA coupled - KLH
    克隆位点
    5E11D8
    亚型
    IgG
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Immunofluorescence (IF): 0.5-2 µg/mL
    Flow cytometry: 0.5-2 µg for 1 x 10^6 cells
    Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized antibody with 100 µL of deionized water to the final concentration of 1 mg/mL.
    浓度
    1 mg/mL
    缓冲液
    PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freezing and thawing cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • 抗原
    HA-Tag
    别名
    HA Tag (HA-Tag 产品)
    物质类
    Tag
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