(7 references)-
- 抗原 See all HA-Tag products
- HA-Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HA-Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 品牌
- THE™
- 序列
- YPYDVPDYA
- 特异性
- THETM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
- 产品特性
- Anti-HA-tag mAb (ABIN387711) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. Anti-HA-tag mAb (Antibodies-Online, ABIN387711) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
- 纯化方法
- Protein A affinity column
- 免疫原
- A KLH coupled synthetic peptide (YPYDVPDYA)
- 克隆位点
- 5E11D8
- 亚型
- IgG1
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anti-HA-Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 18B11H6 unconjugated
anti-HA-Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibodyWB, IF, ChIP 宿主: 兔 Polyclonal RB0411 unconjugated
anti-HA-Tag antibodyWB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP
anti-HA-Tag antibody适用: 标记 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HA-Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 4G8 unconjugated
anti-HA-Tag antibodyELISA, Coat 宿主: 小鼠 Monoclonal 16-43 unconjugated
anti-HA-Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).
: "Novel coronavirus-like particles targeting cells lining the respiratory tract." in: PLoS ONE, Vol. 13, Issue 9, pp. e0203489, (2019) (PubMed).
: "Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro." in: Zygote (Cambridge, England), Vol. 23, Issue 1, pp. 83-92, (2015) (PubMed).
: "Dominant drug targets suppress the emergence of antiviral resistance." in: eLife, Vol. 3, (2014) (PubMed).
: "Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish." in: Zebrafish, Vol. 10, Issue 3, pp. 353-64, (2013) (PubMed).
: "Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35953-63, (2012) (PubMed).
: "Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 28, Issue 5, pp. 1009-22, (2011) (PubMed).
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: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).
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- 抗原
- HA-Tag
- 别名
- HA Tag (HA-Tag 产品)
- 物质类
- Tag
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