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HA-Tag 抗体

Name: HA-Tag 抗体
SKU: ABIN387711
Availability: OutOfStock

Cited in 7+ publications. The 小鼠单克隆 anti-HA-Tag antibody (Clone 5E11D8) (ABIN387711) specifically detects HA-Tag in WB 和 ELISA.

Lane 1-3: GST fusion protein in E. (HA-Tag 抗体)

1 image

(7 references)

产品编号 ABIN387711

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for HA-Tag 抗体 (ABIN387711)

抗原

HA-Tag

宿主

小鼠

克隆类型

单克隆

标记

This HA-Tag antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA

克隆位点

5E11D8

序列

YPYDVPDYA

特异性

THE^TM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.

产品特性

Anti-HA-tag mAb (ABIN387711) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. Anti-HA-tag mAb (Antibodies-Online, ABIN387711) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.

纯化方法

Protein A affinity column

免疫原

A KLH coupled synthetic peptide (YPYDVPDYA)

亚型

IgG1
anti-HA-Tag antibody
WB, ELISA, IP, IF, IHC 宿主: 小鼠 Monoclonal HA-C5 unconjugated

anti-HA-Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 18B11H6 unconjugated

anti-HA-Tag antibody
WB, IF, ChIP 宿主: 兔 Polyclonal RB0411 unconjugated

anti-HA-Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag antibody
WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag (AA 98-106) antibody
Animal-Free 适用: 标记 WB, ELISA, IF 宿主: 兔 Monoclonal AB158-1-D11 unconjugated Recombinant Antibody

anti-HA-Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP

anti-HA-Tag antibody
适用: 标记 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag (AA 98-106) antibody
Animal-Free 适用: 标记 WB, ELISA, IF 宿主: 小鼠 Monoclonal AB158-1-D11 unconjugated Recombinant Antibody

anti-HA-Tag (AA 98-106) antibody
适用: Various Species WB, ELISA, IF, FACS, IHC 宿主: 小鼠 Monoclonal 16-43 unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freezing and thawing cycles.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

Naskalska, Dabrowska, Nowak, Szczepanski, Jasik, Milewska, Ochman, Zeglen, Rajfur, Pyrc: "Novel coronavirus-like particles targeting cells lining the respiratory tract." in: PLoS ONE, Vol. 13, Issue 9, pp. e0203489, (2019) (PubMed).

Cheng, Yu, Liu, Deng, Ma, Wang: "Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro." in: Zygote (Cambridge, England), Vol. 23, Issue 1, pp. 83-92, (2015) (PubMed).

Tanner, Liu, Oberste, Pallansch, Collett, Kirkegaard: "Dominant drug targets suppress the emergence of antiviral resistance." in: eLife, Vol. 3, (2014) (PubMed).

Pillay, Selland, Fleisch, Leighton, Cheng, Famulski, Ritzel, March, Wang, Allison, Waskiewicz: "Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish." in: Zebrafish, Vol. 10, Issue 3, pp. 353-64, (2013) (PubMed).

Shulga, Anderson, Topham, Epand: "Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35953-63, (2012) (PubMed).

Borroto-Escuela, Romero-Fernandez, García-Negredo, Correia, Garriga, Fuxe, Ciruela: "Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 28, Issue 5, pp. 1009-22, (2011) (PubMed).
抗原

HA-Tag

别名

HA Tag

物质类

Tag