DKC1 抗体 (Internal Region)
Quick Overview for DKC1 抗体 (Internal Region) (ABIN374810)
抗原
See all DKC1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-KRKRESESES DETPP
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特异性
- This antibody detects Dyskerin.
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交叉反应 （详细）
- Species reactivity (expected):MouseSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the internal region of the protein sequence according to NP_001354.1 Genename: DKC1
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anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 245-514) antibody
DKC1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) antibodyDKC1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 1-514) antibodyDKC1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 1-180) antibodyDKC1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 1-514) antibodyDKC1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E10 unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 94-170) antibodyDKC1 适用: 人 WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (Internal Region) antibodyDKC1 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 298-465) antibodyDKC1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 298-509) antibodyDKC1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dyskeratosis Congenita 1, Dyskerin (DKC1) (AA 185-213) antibodyDKC1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB26907 unconjugated
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应用备注
- Peptide ELISA: antibody detection limit dilution 1: 128000. Western blot: Preliminary experiments gave an approx 70-75 kDa band in Human BoneMarrow, Duodenum and Skin lysates after 0.01 μg/mL antibody staining. Please note thatcurrently we cannot find an explanation in the literature for the band we observe given thecalculated size of 57.5 kDa according to NP_001354.1. The 70-75 kDa band was successfullyblocked by incubation with the immunizing peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Spleen shows strong nuclear
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))
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别名
- DKC1 / NOLA4
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背景
- DKC1 (Dyskeratosis congenita 1) is a nucleolar protein, altered in dyskeratosis congenita, which carries out two separate functions, both fundamental for proliferating cells. One function is the pseudouridylation of ribosomal RNA (rRNA) molecules, necessary for their processing, and the other is the stabilization of the telomerase RNA component, necessary for telomerase activity. It is thought to be the catalytic subunit of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. DKC1 is also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Both 18S rRNA production and rRNA pseudouridylation are impaired if DKC1 is depleted.Synonyms: CBF5 homolog, Dyskerin, H/ACA ribonucleoprotein complex subunit 4, Nopp140-associated protein of 57 kDa, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1
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基因ID
- 1736
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NCBI登录号
- NP_001354
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UniProt
- O60832
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途径
- Telomere Maintenance
抗原
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