PCK1 抗体 (C-Term, Internal Region)
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Quick Overview for PCK1 抗体 (C-Term, Internal Region) (ABIN374797)
抗原
See all PCK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term, Internal Region
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序列
- C-EKEVEDIEKY LEDQ
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特异性
- This antibody detects PEP carboxykinase.
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交叉反应 （详细）
- Species reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the internal region (near the C Terminus) of the protein sequence according to NP_002582.2. Genename: PCK1
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anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 592-622), (C-Term) antibody
PCK1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal RB03646 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibodyPCK1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 312-563) antibodyPCK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibodyPCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 330-379) antibodyPCK1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 猴, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 40-70), (N-Term) antibodyPCK1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3644 unconjugated
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应用备注
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Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.5-1.5 μg/mL. Approx 70 kDa band observed in Human Kidney lysates(calculated MW of 69.0 kDa according to NP_002582.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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别名
- PCK1 / PEPCK1
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背景
- PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.Synonyms: PEP carboxykinase, PEPCK-C, PEPCKC, Phosphoenolpyruvate carboxykinase cytosolic [GTP], Phosphoenolpyruvate carboxylase
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基因ID
- 5105
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NCBI登录号
- NP_002582
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UniProt
- P35558
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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