NCF4 抗体 (Internal Region)
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Quick Overview for NCF4 抗体 (Internal Region) (ABIN374770)
抗原
See all NCF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody reacts to NCF4 / P40PHOX.
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交叉反应 (详细)
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Species reactivity (expected):Canine.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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免疫原
- Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence
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应用备注
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Peptide ELISA: 1/32000. Western blot: Approx.38 kDa band observed in lysates of cell line Daudi (calculated MW of39.8 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). Intransfected HEK293 transiently expressing P40PHOX a band of approx.48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Tonsil shows staining of thecytoplasm in some cells. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
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别名
- NCF-4
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背景
- NCF4 is a cytosolic oxidase associated protein. It is a component of the nicotinamide adenine dinucleotide phosphate oxidase, which mediates down regulation of NADPH oxidase through interactions with its SH3 domain. NCF4 is associated with p67 phox but is absent in patients with chronic granulomatous disease who lack p67 phox.Synonyms: Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4, SH3PXD4, p40-phox
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基因ID
- 4689
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NCBI登录号
- NP_000622
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UniProt
- Q15080
抗原
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