Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 35 kDa band observed in human kidney and humanliver lysates (calculated MW of 35.5 kDa according to NP_002027.2). Immunohistochemistry: 3 μg/mL. In paraffin embedded human kidney, stains epithelialcells of renal tubules and endothelial cells of vessels. The granular staining is compatiblewith the endoplasmic reticulum association of the protein. This antibody has also beensuccessfully used on Huamn Lung showing endothelial staining. FACS: A customer has reported Successful staining of HEK293S-TetR transfected with DARCreceptor at a dilution of 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system.Synonyms: Duffy antigen/chemokine receptor, FY, Fy glycoprotein, GPD, Glycoprotein D, GpFy, Plasmodium vivax receptor