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Osteopontin 抗体 (C-Term)

This 山羊 多克隆 antibody specifically detects Osteopontin in WB, IHC (p) 和 EIA. It exhibits reactivity toward 人, 小鼠, 大鼠 和 犬.
产品编号 ABIN374581
发货至: 中国
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Quick Overview for Osteopontin 抗体 (C-Term) (ABIN374581)

抗原

See all Osteopontin (SPP1) 抗体
Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

适用

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人, 小鼠, 大鼠, 犬

宿主

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山羊

克隆类型

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多克隆

标记

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This Osteopontin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    C-Term

    序列

    C-DPKSKEEDKH LK

    特异性

    Recognizes Osteopontin (SPP1).

    交叉反应 (详细)

    Species reactivity (expected):Canine (Dog), Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

    免疫原

    Synthetic peptide KLH conjugated corresponding to C-Terminus of Osteopontin. Genename: SPP1
  • 应用备注

    Peptide ELISA: 1/64000. Western Blot: 1 - 3 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline buffer, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

    别名

    Osteopontin / SPP1

    背景

    Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.Synonyms: BNSP, Bone Sialoprotein 1, Nephropontin, OPN, SPP-1, Secreted Phosphoprotein 1, Urinary Stone Protein, Uropontin

    基因ID

    6696, 9606

    UniProt

    P10451

    途径

    Regulation of Cell Size
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