PPY 抗体 (Internal Region)
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北京 101111
Quick Overview for PPY 抗体 (Internal Region) (ABIN374577)
抗原
See all PPY 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-TRPRYGKRHK EDT
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特异性
- This antibody recognizes Pancreatic prohormone.
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交叉反应 （详细）
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Species reactivity (expected):Rat and Canine (Dog).
Species reactivity (tested):Human, Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Synthetic peptide from the internal region of the protein sequence according to NP_002713.1. Genename: PPY
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anti-Pancreatic Polypeptide (PPY) (AA 1-95) antibody
PPY 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2G5D12 unconjugated
anti-Pancreatic Polypeptide (PPY) (Internal Region) antibodyVerified PPY 适用: 人, 大鼠 WB, IHC, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Pancreatic Polypeptide (PPY) (AA 1-95) antibodyPPY 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Pancreatic Polypeptide (PPY) (AA 31-97) antibodyPPY 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic Polypeptide (PPY) (AA 30-87) antibodyPPY 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic Polypeptide (PPY) antibodyPPY 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic Polypeptide (PPY) (AA 31-97) antibodyPPY 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic Polypeptide (PPY) (AA 31-97) antibodyPPY 适用: 小鼠 WB, IHC, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Antibody Detection Limit Dilution: 1/32000. Western Blot: Preliminary experiments gave an approx 35-38 kDa band in human pancreaslysates after 1 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 10.8 kDaaccording to NP_002713.1. The 38 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3 μg/mL. In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cellslocated at the periphery of islets of Langerhans.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PPY (Pancreatic Polypeptide (PPY))
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别名
- Pancreatic Prohormone
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背景
- Pancreatic hormone (PPY) is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.Synonyms: Obinepitide, PNP, PPY, Pancreatic polypeptide
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基因ID
- 5539
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NCBI登录号
- NP_002713
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UniProt
- P01298
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途径
- Feeding Behaviour
抗原
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