LIME 抗体 (C-Term)
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Quick Overview for LIME 抗体 (C-Term) (ABIN374438)
抗原
See all LIME (LIME1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-ENVYESIKEM GL
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特异性
- This antibody recognizes LIME1.
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交叉反应 （详细）
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Species reactivity (expected):Rat.
Species reactivity (tested):Mouse -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide from the C Terminus of the protein sequence according to NP_076173.1.
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anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibody
LIME1 适用: 人 IP, ICFC 宿主: 小鼠 Monoclonal LIME-06 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 170-295) antibodyLIME1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 281-296) antibodyLIME1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal LIME-10 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibodyLIME1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (C-Term) antibodyVerified LIME1 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 86-202) antibodyLIME1 适用: 小鼠 WB, IP 宿主: 小鼠 Monoclonal mLIME-05 unconjugated
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应用备注
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Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL, Approx 32 kDa band observed in Mouse Heart lysates(predicted MW of 32 kDa according to NP_076173.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
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别名
- LIME1
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背景
- LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1
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基因ID
- 54923
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NCBI登录号
- NP_060276
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UniProt
- Q9H400
抗原
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