BAF53A 抗体 (C-Term)
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Quick Overview for BAF53A 抗体 (C-Term) (ABIN374427)
抗原
See all BAF53A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- YEEGGKQCVE RKCP
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特异性
- This antibody detects ACTL6A / BAF53 at C-term.
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交叉反应 （详细）
- Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1. Genename: ACTL6A / ACTL6B
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anti-Actin-Like 6A (BAF53A) antibody
BAF53A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Actin-Like 6A (BAF53A) antibodyKD Validated BAF53A 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 23GB1155 unconjugated Recombinant Antibody
anti-Actin-Like 6A (BAF53A) (AA 295-320), (C-Term) antibodyBAF53A 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB51048 unconjugated
anti-Actin-Like 6A (BAF53A) (AA 1-80) antibodyBAF53A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C4 unconjugated
anti-Actin-Like 6A (BAF53A) (AA 1-280) antibodyBAF53A 适用: 人 WB, IHC, ELISA, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Actin-Like 6A (BAF53A) (AA 25-75) antibodyBAF53A 适用: 人, 小鼠 WB, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Actin-Like 6A (BAF53A) (Internal Region) antibodyBAF53A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin-Like 6A (BAF53A) antibodyBAF53A 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin-Like 6A (BAF53A) antibodyKD Validated BAF53A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Actin-Like 6A (BAF53A) (Center) antibodyKO Validated, KD Validated BAF53A 适用: 人, 小鼠, 大鼠, Cow, 犬, 小鸡, Pig WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 64000. Western Blot: 1-3 μg/mL. Approx 48 kDa band observed in Hela lysates (predicted MW of48 kDa according to NP_057272, NP_004292).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- BAF53A (Actin-Like 6A (BAF53A))
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别名
- ACTL6A / BAF53
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背景
- ACTL6A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila, the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. ACTL6A is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.Synonyms: 53 kDa BRG1-associated factor A, Actin-like protein 6A, Actin-related protein Baf53a, ArpNbeta, BAF53A, INO80 complex subunit K, INO80K
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基因ID
- 86
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NCBI登录号
- NP_004292
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UniProt
- O96019
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途径
- Chromatin Binding, Photoperiodism
抗原
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