MPG 抗体 (C-Term)
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Quick Overview for MPG 抗体 (C-Term) (ABIN374423)
抗原
See all MPG 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-SVVDRVAEQD TQA
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特异性
- This antibody detects MPG / ANPG at C-term.
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交叉反应 (详细)
- Species reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_002425.2, NP_001015052.1, NP_001015054.1. Genename: MPG
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应用备注
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 28-30 kDa band observed in lysates of cell line K562(calculated MW of 30.0 kDa according to NP_001015054.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- MPG (N-Methylpurine-DNA Glycosylase (MPG))
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别名
- MPG / ANPG
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背景
- MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.Synonyms: 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, AAG, ADPG, DNA-3-methyladenine glycosylase, MID1, N-methylpurine-DNA glycosylase
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基因ID
- 4350
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NCBI登录号
- NP_002425
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UniProt
- P29372
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途径
- DNA Damage Repair
抗原
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