AKAP8 抗体 (N-Term)
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Quick Overview for AKAP8 抗体 (N-Term) (ABIN374421)
抗原
See all AKAP8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- DQGYGGYGAW SAG-C
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特异性
- This antibody detects AKAP8 at N-term.
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交叉反应 (详细)
- Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the N Terminus of the protein sequence according to NP_005849.1. Genename: AKAP8
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Experiments gave a band at approx 100 kDa in Human Liver lysateafter 1 μg/mL antibody staining. This band corresponds to the 90 kDa band reported inliterature (PMID: 10601332). This protein has a calculated MW of 76.0 kDa according toNP_005849.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- AKAP8 (A Kinase (PRKA) Anchor Protein 8 (AKAP8))
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别名
- AKAP8
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背景
- The A kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This antibody recognises a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.Synonyms: A-kinase anchor protein 8, A-kinase anchor protein 95 kDa, AKAP95
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基因ID
- 10270
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NCBI登录号
- NP_005849
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UniProt
- O43823
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途径
- SARS-CoV-2 Protein Interactome
抗原
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