RANGAP1 抗体 (N-Term)
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Quick Overview for RANGAP1 抗体 (N-Term) (ABIN374398)
抗原
See all RANGAP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- ASEDIAKLAE TLAK-C
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特异性
- This antibody detects RANGAP1 at N-term.
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交叉反应 (详细)
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Species reactivity (expected):Rat, Dog, Pig.
Species reactivity (tested):Human, Mouse. -
纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from N Terminus of the protein sequence according to NP_002874.1 Genename: RANGAP1
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应用备注
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Peptide ELISA: 1/32000. Western blot: Approx 70 kDa band observed in Human Brain and 3T3 lysates (predicted MWof 60 kDa according to NP_002874). Recommended concentration: 0.1 - 0.5 μg/mL. Immunohistochemistry on paraffin sections: Human Liver shows vesicular staining in thecytoplasm of hepatocytes. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))
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别名
- RanGAP1
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背景
- Synonyms: KIAA1835, Ran GTPase-activating protein 1, SD, segregation distorter homolog
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基因ID
- 5905
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NCBI登录号
- NP_002874
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UniProt
- P46060
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途径
- M Phase, Protein targeting to Nucleus
抗原
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