POLL 抗体 (C-Term)
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Quick Overview for POLL 抗体 (C-Term) (ABIN374383)
抗原
See all POLL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-LGLPYREPAE RDW
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特异性
- This antibody detects DNA Polymerase Lambda at C-term.
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交叉反应 （详细）
- Species reactivity (expected):MouseSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_037406.1 Genename: POLL
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anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibody
Verified POLL 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 525-575) antibodyPOLL 适用: 人, 小鼠 IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 525-575) antibodyPOLL 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 16-211) antibodyPOLL 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (N-Term) antibodyPOLL 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (AA 150-200) antibodyPOLL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), lambda (POLL) (C-Term) antibodyPOLL 适用: 人, 小鼠, 兔, 豚鼠, Pig, 犬, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave a band at approx 40 kDa in Human Liver andHepG2 extracts at 1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Small Intestine showsnuclear staining of enterocytes and of most cells in the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- POLL (Polymerase (DNA Directed), lambda (POLL))
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别名
- DNA Polymerase lambda
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背景
- DNA Polymerase lambda is a family X member repair polymerase with low frameshift fidelity that is involved in base excision repair (BER) and is responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Polymerase lambda is the primary gap filling polymerase for accurate nonhomologous end joining, and the Brca1 C terminal domain is required for this activity.Synonyms: DNA polymerase beta-2, DNA polymerase kappa, POLL, Pol Lambda
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基因ID
- 27343
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NCBI登录号
- NP_037406
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UniProt
- Q9UGP5
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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