STRADB 抗体 (C-Term)
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Quick Overview for STRADB 抗体 (C-Term) (ABIN374305)
抗原
See all STRADB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody detects STRAD beta / ILPIP at C-term. This is expected to recognise both human isoforms: ILPIP-alpha and ILPIP-beta.
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交叉反应 (详细)
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Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human -
纯化方法
- Affinity chromatography
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免疫原
- Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence Genename: STRADB
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应用备注
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Peptide ELISA: 1/32000. Western blot: 1 - 3 μg/mL. Approx 35 kDa band observed in Human Heart, Liver and Musclelysates (calculated MW of 47.5 kDa according to NP_061041.2 (alpha isoform) and 31.5 kDaaccording to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIPa band of approx.45 kDa is observed. This band is not observed in the non-transfectedHEK293. Immunohistochemistry on paraffin sections: 3 - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- STRADB (STE20-Related Kinase Adaptor beta (STRADB))
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别名
- STRAD beta / ILPIP
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背景
- Synonyms: ALS2CR2, CALS-21, PRO1038, Pseudokinase ALS2CR2, STE20-related kinase adapter protein beta, STRADB
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基因ID
- 55437
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NCBI登录号
- NP_061041
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UniProt
- Q9C0K7
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途径
- AMPK Signaling
抗原
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