RAB11A 抗体 (C-Term)
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Quick Overview for RAB11A 抗体 (C-Term) (ABIN374252)
抗原
See all RAB11A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- TENKPKVQCC QNI
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特异性
- This antibody detetcs RAB11A at C-term.
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交叉反应 (详细)
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Species reactivity (expected):Human, Rat, Cow.
Species reactivity (tested):Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide from the C Terminus of the protein sequence according to NP_004654.1 Genename: RAB11A
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应用备注
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Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 26 kDa band observed in Mouse Heart lysates (CalculatedMW of 24.6 kDa according to NP_004654.1). In transfected HEK293 transiently expressingRAB11A a band of approx.26 kDa is observed. This band is not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))
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别名
- RAB11A / RAB11
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背景
- Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.Synonyms: RAB-11, Ras-related protein Rab-11A, YL8
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基因ID
- 8766
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NCBI登录号
- NP_004654
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UniProt
- P62491
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途径
- Regulation of Cell Size, Thromboxane A2 Receptor Signaling, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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