UBE2C 抗体 (C-Term)
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- 抗原 See all UBE2C 抗体
- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This UBE2C antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 序列
- C-QETYSKQVTS QEP
- 特异性
- This antibody is expected to recognise all reported isoforms.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide from C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1. Genename: UBE2C
- Top Product
- Discover our top product UBE2C Primary Antibody
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anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 70-179) antibody
UBE2C 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 9D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1F5D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (C-Term) antibodyVerified UBE2C 适用: 人 WB, ELISA, IF, IP, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approximately 20KDa band was detected in mitotic (i. eNocodazole arrested) HeLa whole cell lysates. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates usingprotein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, withvery faint or no signal in G1 and S phase cells (fixed using paraformaldehyde, primaryconcentration 2.5 μg/mL, using FITC anti-Goat).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
- 别名
- UBE2C / UBCH10 (UBE2C 产品)
- 别名
- LOC458287 antibody, UBCH10 antibody, dJ447F3.2 antibody, 1110015A16Rik antibody, D2Ertd695e antibody, im:7137135 antibody, zgc:123190 antibody, ubiquitin conjugating enzyme E2 C antibody, ubiquitin-conjugating enzyme E2C antibody, ubiquitin conjugating enzyme E2 C L homeolog antibody, ubiquitin conjugating enzyme E2 C S homeolog antibody, UBE2C antibody, NAEGRDRAFT_82946 antibody, ube2c.L antibody, Ube2c antibody, ube2c.S antibody, ube2c antibody
- 背景
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.Synonyms: Ubiquitin carrier protein C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C
- 基因ID
- 11065
- NCBI登录号
- NP_008950
- UniProt
- O00762
- 途径
- Ubiquitin Proteasome Pathway
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