UBE2C 抗体 (C-Term)
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Quick Overview for UBE2C 抗体 (C-Term) (ABIN374251)
抗原
See all UBE2C 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-QETYSKQVTS QEP
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特异性
- This antibody is expected to recognise all reported isoforms.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1. Genename: UBE2C
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anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 70-179) antibody
UBE2C 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 9D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F5D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (C-Term) antibodyVerified UBE2C 适用: 人 WB, ELISA, IF, IP, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 25-129) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D8 unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approximately 20KDa band was detected in mitotic (i. eNocodazole arrested) HeLa whole cell lysates. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates usingprotein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, withvery faint or no signal in G1 and S phase cells (fixed using paraformaldehyde, primaryconcentration 2.5 μg/mL, using FITC anti-Goat).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
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别名
- UBE2C / UBCH10
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背景
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.Synonyms: Ubiquitin carrier protein C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C
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基因ID
- 11065
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NCBI登录号
- NP_008950
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UniProt
- O00762
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途径
- Ubiquitin Proteasome Pathway
抗原
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