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UBE2C 抗体 (C-Term)

Verified UBE2C 适用: 人 WB, ELISA, IF, IP, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184805
发货至: 中国
  • 抗原 See all UBE2C 抗体
    UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
    抗原表位
    • 15
    • 10
    • 7
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 75
    • 14
    • 13
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    宿主
    • 63
    • 12
    山羊
    克隆类型
    • 61
    • 14
    多克隆
    标记
    • 35
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This UBE2C antibody is un-conjugated
    应用范围
    • 62
    • 37
    • 17
    • 13
    • 13
    • 10
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)
    原理
    UBE2C / UBCH10
    序列
    QETYSKQVTS QEP
    特异性
    This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
    交叉反应
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.
    亚型
    IgG
    Top Product
    Discover our top product UBE2C Primary Antibody
  • 应用备注

    Western Blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
    别名
    UBE2C (UBE2C 产品)
    别名
    LOC458287 antibody, UBCH10 antibody, dJ447F3.2 antibody, 1110015A16Rik antibody, D2Ertd695e antibody, im:7137135 antibody, zgc:123190 antibody, ubiquitin conjugating enzyme E2 C antibody, ubiquitin-conjugating enzyme E2C antibody, ubiquitin conjugating enzyme E2 C L homeolog antibody, ubiquitin conjugating enzyme E2 C S homeolog antibody, UBE2C antibody, NAEGRDRAFT_82946 antibody, ube2c.L antibody, Ube2c antibody, ube2c.S antibody, ube2c antibody
    背景
    UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1
    基因ID
    11065
    NCBI登录号
    NP_008950, NP_861515, NP_861516, NP_861517, NP_861518, NP_001268670
    途径
    Ubiquitin Proteasome Pathway
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