NQO1 抗体 (C-Term)
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北京 101111
Quick Overview for NQO1 抗体 (C-Term) (ABIN374187)
抗原
See all NQO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-SIPTDNQIKA RK
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特异性
- This antibody is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1) of NQO1.
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交叉反应 （详细）
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Species reactivity (expected):Rat, Pig.
Species reactivity (tested):Human, Mouse, Canine. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_000894.1, NP_001020604.1, NP_001020605.1. Genename: NQO1
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anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) antibody
NQO1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4D12 unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (AA 2-274) antibodyNQO1 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (C-Term) antibodyVerified NQO1 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (AA 1-274) antibodyNQO1 适用: 人 WB, ELISA, IF, IP, RNAi 宿主: 小鼠 Monoclonal 1E3-A6 unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (AA 1-274) antibodyNQO1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (C-Term) antibodyNQO1 适用: 人, 小鼠, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (AA 2-273) antibodyNQO1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (AA 2-274) antibodyNQO1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (AA 1-50) antibodyNQO1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (AA 118-144) antibodyNQO1 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx.30 kDa band observed in Human Kidney and Lunglysates (calculated MW of 30.0 kDa according to NP_000894.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "ANIT-induced intrahepatic cholestasis alters hepatobiliary transporter expression via Nrf2-dependent and independent signaling." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 108, Issue 2, pp. 247-57, (2009) (PubMed).
: "Sensory neurons and schwann cells respond to oxidative stress by increasing antioxidant defense mechanisms." in: Antioxidants & redox signaling, Vol. 11, Issue 3, pp. 425-38, (2009) (PubMed).
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- NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))
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别名
- NQO1
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背景
- NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. Mutations in the gene for NQO1 have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). NQO1 levels have been shown to be raised in may tumour types. This has led to a search for anti-tumour agents that can be activated by NQO1 resulting in specific targetting of these compounds to the tumour site. Many cytotoxic drugs contain the quinone moiety, such as the benzoquinone group, which can be activated by NQO1 through the reduction into hydroquinone [which in this case can auto-oxidize and lead to the formation of free radicals and alkylating species]. However, the rat enzyme has been shown to be significantly more effective in activating some of these compounds than the human and mouse enzymes. This indicates that the available cytotoxic drugs are better substrates for the rat enzyme and are not the most ideal prodrugs for activation by NQO1 in human tumors.Synonyms: Azoreductase, DIA4, DT-diaphorase, Menadione reductase, NAD(P)H dehydrogenase [quinone] 1, NAD(P)H:quinone oxidoreductase 1, NMOR1, Phylloquinone reductase, Quinone reductase 1
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基因ID
- 1728
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NCBI登录号
- NP_000894
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UniProt
- P15559
抗原
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