RACGAP1 抗体 (C-Term)
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Quick Overview for RACGAP1 抗体 (C-Term) (ABIN374167)
抗原
See all RACGAP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-GRQGNFFASP MLK
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特异性
- This antibody detects RACGAP1 at C-term.
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交叉反应 (详细)
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Species reactivity (expected):Canine (Dog) and Bovine.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1. Genename: RACGAP1
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应用备注
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Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 60 kDa band observed in Human Testis lysates(calculated MW of 71.0 kDa according to NP_037409.2). The observed molecular weightcorresponds to earlier findings in literature with different antibodies (Toure et al. , J BiolChem 1998 Mar 13,273(11): 6019-23, PMID: 9497316). Immunohistochemistry: 1 μg/mL. In paraffin embedded Human Testis, shows nuclearstaining of many cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))
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别名
- RACGAP1
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背景
- Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.Synonyms: KIAA1478, MGCRACGAP, Male germ cell RacGap, Rac GTPase-activating protein 1
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分子量
- 71kDa (NP_037409).
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基因ID
- 29127
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NCBI登录号
- NP_037409
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UniProt
- Q9H0H5
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途径
- Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor
抗原
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