CrkL 抗体 (C-Term)
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Quick Overview for CrkL 抗体 (C-Term) (ABIN374141)
抗原
See all CrkL (CRKL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-KIFDPQNPDE NE
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特异性
- This antibody detects CRLK at C-term.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_005198.1 Genename: CRKL
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anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) antibody
CRKL 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 204-303) antibodyCRKL 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 4B5 unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibodyCRKL 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5E12 unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (pTyr251) antibodyCRKL 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) antibodyCRKL 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 201-303) antibodyCRKL 适用: 小鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) antibodyCRKL 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 98-303) antibodyCRKL 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (C-Term) antibodyVerified CRKL 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibodyKD Validated CRKL 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB4735 unconjugated Recombinant Antibody
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应用备注
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.01-0.03 μg/mL. Approx 37 kDa band observed in K562 and Jurkat lysates(calculated MW of 33.7 kDa according to NP_005198).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
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别名
- CRKL
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背景
- Crkl is a 33 kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.Synonyms: Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like
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基因ID
- 1399
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NCBI登录号
- NP_005198
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UniProt
- P46109
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途径
- Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor
抗原
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