Cortactin 抗体 (N-Term)
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Quick Overview for Cortactin 抗体 (N-Term) (ABIN374140)
抗原
See all Cortactin (CTTN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- WKASAGHAVS IA-C
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特异性
- This antibody detects Cortactin at N-term.
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from N Terminus of the protein sequence according to NP_005222.2, NP_612632.1 Genename: CTTN
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anti-Cortactin (CTTN) (N-Term) antibody
KO Validated CTTN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 214-513) antibodyCTTN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Cortactin (CTTN) (pTyr466) antibodyCTTN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) antibodyKD Validated CTTN 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB2335 unconjugated Recombinant Antibody
anti-Cortactin (CTTN) (AA 341-450) antibodyCTTN 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6 unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 1-200) antibodyCTTN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx.80 kDa band observed in Mouse and Rat Brainlysates (calculated MW of 61.0 kDa according to Human NP_005222.2 and of 61.0 kDaaccording to Mouse NP_031829.2). The observed molecular weight corresponds to earlierfindings in literature with different antibodies (Paradis et al, J Cell Sci.2008 Jun 15,121(Pt12): 1965-72, PMID 18495841).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Cortactin (CTTN)
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别名
- Cortactin
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背景
- Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1
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基因ID
- 2017
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NCBI登录号
- NP_005222
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UniProt
- Q14247
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途径
- MAPK Pathway
抗原
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