SMAD4 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for SMAD4 抗体 (C-Term) (ABIN374104)
抗原
See all SMAD4 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
序列
- C-HTMPIADPQP LD
-
特异性
- This antibody detects SMAD4 at C-term.
-
交叉反应 （详细）
-
Species reactivity (expected):Human, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
-
免疫原
- Peptide from C-Terminus of the protein sequence according to NP_005350.1 Genename: SMAD4
-
-
anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody
SMAD4 适用: 人 WB, IP, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 1-552) antibodySMAD4 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 3E2 unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodySMAD4 适用: 人, 小鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4G1C6 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 255-284) antibodySMAD4 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB14714 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 400-428) antibodySMAD4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 27-195) antibodySMAD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodyKD Validated SMAD4 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 23GB975 unconjugated Recombinant Antibody
anti-SMAD Family Member 4 (SMAD4) (AA 400-428), (C-Term) antibodySMAD4 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB20720 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 40-120) antibodySMAD4 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodySMAD4 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 3A1 unconjugated
-
-
应用备注
-
Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 60-65 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- SMAD4 (SMAD Family Member 4 (SMAD4))
-
别名
- SMAD4
-
背景
- SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-beta (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus. Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4
-
基因ID
- 4089
-
NCBI登录号
- NP_005350
-
UniProt
- Q13485
-
途径
- Cell Division Cycle, Chromatin Binding, Autophagy
抗原
-
