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Aurora A 抗体 (C-Term)

This 山羊 多克隆 antibody specifically detects Aurora A in WB. It exhibits reactivity toward 人.
产品编号 ABIN374071
发货至: 中国
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Quick Overview for Aurora A 抗体 (C-Term) (ABIN374071)

抗原

See all Aurora A (AURKA) 抗体
Aurora A (AURKA) (Aurora Kinase A (AURKA))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Aurora A antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

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    C-Term

    序列

    C-QNKESASKQS

    特异性

    This antibody detects Aurora kinase A / AURKA at C-term.

    纯化方法

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    免疫原

    Peptide from the C Terminus of the protein sequence according to NP_003591.2.
  • 应用备注

    Peptide ELISA: Detection limit dilution 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 45 kDa band observed in lysates of cell line HeLa andJurkat lysate (calculated MW of 45.5 kDa according to NP_003591.2). In transfected HEK293transiently expressing AURKA a band of approx.45 kDa is observed. This band is notobserved in the non-transfected HEK293.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Aurora A (AURKA) (Aurora Kinase A (AURKA))

    别名

    Aurora Kinase A

    背景

    Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

    基因ID

    6790

    NCBI登录号

    NP_003591

    UniProt

    O14965

    途径

    Cell Division Cycle, Asymmetric Protein Localization
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