MIF 抗体 (C-Term)
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Quick Overview for MIF 抗体 (C-Term) (ABIN372861)
抗原
See all MIF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody recognizes Macrophage Migration Inhibitory Factor (MIF).
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Synthetic peptide (C-NAANVGWNNSTFA) from C-terminus of Human MIF
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亚型
- IgG
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anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 3-111) antibody
MIF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) antibodyMIF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-115) antibodyMIF 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 2A10-4D3 unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) antibodyMIF 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) antibodyMIF 适用: 人 WB, ELISA, IHC (p), IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 51-115) antibodyMIF 适用: 人, 大鼠 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibodyVerified MIF 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) antibodyMIF 适用: 人 WB, IHC, FACS, ICC 宿主: 兔 Monoclonal BLR141J unconjugated Recombinant Antibody
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Internal Region) antibodyMIF 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) antibodyMIF 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/128000. Western blot: 0.01-0.03 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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别名
- MIF
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背景
- MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
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基因ID
- 4282
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NCBI登录号
- NP_002406
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UniProt
- P14174
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
抗原
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