GAPDH 抗体 (AA 73-87)
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Quick Overview for GAPDH 抗体 (AA 73-87) (ABIN372846)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 73-87
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特异性
- This antibody reacts to Glyceraldehyde 3-phosphate Dehydrogenase (GAPDH).
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein
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亚型
- IgG
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibodyGAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibodyVerified GAPDH 适用: 人, 大鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibodyGAPDH 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 斑马鱼 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibodyGAPDH 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on paraffin sections: 5 μg/mL. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS conatining 0.05 % BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
- Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
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基因ID
- 2597
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NCBI登录号
- NP_002037
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UniProt
- P04406
抗原
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