Osteopontin 抗体 (C-Term)
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Quick Overview for Osteopontin 抗体 (C-Term) (ABIN372777)
抗原
See all Osteopontin (SPP1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- DPKSKEEDKH LK
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特异性
- Recognizes Osteopontin (SPP1).
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交叉反应 (详细)
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Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Synthetic peptide KLH conjugated corresponding to C-Terminus of Osteopontin.
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亚型
- IgG
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应用备注
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ELISA: 1/64000. Western Blot: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- Osteopontin / SPP1
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背景
- Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.Synonyms: BNSP, Bone Sialoprotein 1, Nephropontin, OPN, SPP-1, Secreted Phosphoprotein 1, Urinary Stone Protein, Uropontin
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基因ID
- 6696
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NCBI登录号
- NP_000573
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UniProt
- P10451
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途径
- Regulation of Cell Size
抗原
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