NMDAR2A 抗体 (C-Term)
(6 references)-
- 抗原 See all NMDAR2A (GRIN2A) 抗体
- NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))
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抗原表位
- C-Term
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NMDAR2A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognizes the ~180k NR2A subunit of the NMDA Receptor in Western Blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Mouse and Rat.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Fusion protein from the C-terminus of the NR2A subunit of Rat NMDA Receptor.
- 亚型
- IgG
- Top Product
- Discover our top product GRIN2A Primary Antibody
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anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 851-950) antibody
GRIN2A 适用: 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 501-750) antibodyGRIN2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 1130-1400) antibodyGRIN2A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) antibodyGRIN2A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 23-165) antibodyGRIN2A 适用: 人 WB, IHC, ELISA, ICC, FACS, Neut 宿主: 小鼠 Monoclonal 3G10F9 unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 75-325) antibodyGRIN2A 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S327-95 unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 75-325) antibodyGRIN2A 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S327A-38 unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 75-325) antibody (HRP)GRIN2A 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S327-95 HRP
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 75-325) antibody (HRP)GRIN2A 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S327A-38 HRP
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 75-325) antibody (FITC)GRIN2A 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S327-95 FITC
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- 应用备注
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Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000. Immunoprecipitation: 3 μL per 200 μg lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
- 缓冲液
- 5 mM Ammonium Bicarbonate.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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: "Rescue of NMDAR-dependent synaptic plasticity in Fmr1 knock-out mice." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 25, Issue 1, pp. 271-9, (2014) (PubMed).
: "Dual leucine zipper kinase is required for excitotoxicity-induced neuronal degeneration." in: The Journal of experimental medicine, Vol. 210, Issue 12, pp. 2553-67, (2013) (PubMed).
: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).
: "Modulation of NR2B-regulated contextual fear in the hippocampus by the tissue plasminogen activator system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 33, pp. 13473-8, (2007) (PubMed).
: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, (2003) (PubMed).
: "Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11020-5, (2003) (PubMed).
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: "Rescue of NMDAR-dependent synaptic plasticity in Fmr1 knock-out mice." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 25, Issue 1, pp. 271-9, (2014) (PubMed).
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- 抗原
- NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))
- Abstract
- GRIN2A 产品
- 别名
- NMDAR2A antibody, nr2a antibody, GRIN2A antibody, EPND antibody, GluN2A antibody, NR2A antibody, glutamate ionotropic receptor NMDA type subunit 2A antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog antibody, glutamate receptor ionotropic, NMDA 2A antibody, glutamate receptor, ionotropic, NMDA2A (epsilon 1) antibody, GRIN2A antibody, grin2a.L antibody, LOC100438553 antibody, Grin2a antibody
- 背景
- The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.Synonyms: GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, NMDAR2A, NR2A
- 基因ID
- 2903
- NCBI登录号
- NP_000824
- UniProt
- Q12879
- 途径
- Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity
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