Fibrillarin 抗体
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北京 101111
Quick Overview for Fibrillarin 抗体 (ABIN371824)
抗原
See all Fibrillarin (FBL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for the ~34 kDa Fibrillarin /Nop1p protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
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交叉反应 （详细）
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Species reactivity (expected):Mammals.
Species reactivity (tested):Human, Rat, Plant, Drosophila, C. elegans and S. pombe. -
产品特性
- Synonyms: FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1
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纯化方法
- Ig Fraction
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免疫原
- Yeast nuclear preparations.
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亚型
- IgG1
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anti-Fibrillarin (FBL) antibody
FBL 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) (AA 100-321) antibodyFBL 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL 适用: Saccharomyces cerevisiae WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 38F3 unconjugated
anti-Fibrillarin (FBL) (AA 1-321) antibodyFBL 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4G9-E4 unconjugated
anti-Fibrillarin (FBL) (N-Term) antibodyFBL 适用: 人, 大鼠, 小鼠, 犬, Cow, 兔, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 23GB5735 unconjugated Recombinant Antibody
anti-Fibrillarin (FBL) (Internal Region) antibodyFBL 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) (AA 61-110) antibodyFBL 适用: 人, 大鼠, 小鼠, 犬, Cow, 猴, 豚鼠, 马 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
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Store the antibody undiluted (in aliquots) at -20 °C.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Fibrillarin (FBL)
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别名
- FBL
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背景
- Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3).Synonyms: FIB1, FLRN, Fibrillarin, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin
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基因ID
- 851548
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UniProt
- P15646
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途径
- Ribonucleoside Biosynthetic Process
抗原
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