MAP1LC3A 抗体 (AA 50-150)
Quick Overview for MAP1LC3A 抗体 (AA 50-150) (ABIN363516)
抗原
See all MAP1LC3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 50-150
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特异性
- Reacts with LC3 from human, mouse, rat and Zebrafish
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交叉反应
- 牛
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交叉反应 (详细)
- Predicted to react with Xenopus and bovine based on 100 % sequence homology.
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纯化方法
- Antigen affinity purified
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免疫原
- Synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150)
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应用备注
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Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ICC/IF1:100-1:300, IHC1:200-1:400, IHC-P1:200-1:400, IP20 μL / 500 μg of lysate, WB2 μg/mL -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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- MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
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别名
- Light Chain 3 (LC3)
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背景
- Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
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基因ID
- 84557
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途径
- Autophagy
抗原
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