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EIF2A 抗体 (AA 49-53)

There is 1 publication for this product available. The 兔 多克隆 anti-EIF2A antibody is suitable to detect EIF2A in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IHC 和 IF.
产品编号 ABIN362970
发货至: 中国
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Quick Overview for EIF2A 抗体 (AA 49-53) (ABIN362970)

抗原

See all EIF2A 抗体
EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

适用

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人, 小鼠, 大鼠

宿主

  • 98
  • 15
  • 2

克隆类型

  • 66
  • 49
多克隆

标记

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This EIF2A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

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    AA 49-53

    特异性

    The antibody detects endogenous level of total eIF2a protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

    免疫原

    Peptide sequence around AA 49-53 (E-L-S-R-R) derived from Human eIF2a. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Wu, Li, Lin, Wang, Li, Tie, Song, Guo, Yu, Li: "Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction." in: Cardiovascular research, Vol. 81, Issue 1, pp. 148-58, (2008) (PubMed).

  • 抗原

    EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

    别名

    eIF2alpha

    背景

    Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

    分子量

    38 kDa

    NCBI登录号

    NP_004085

    UniProt

    P05198

    途径

    Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
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