The antibody detects endogenous level of total Paxillin protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
Peptide sequence around AA 29-33 (T-P-Y-S-Y) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
抗原
Paxillin (PXN)
别名
Paxillin
背景
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions