Cytokeratin 18 抗体 (pSer33)
Quick Overview for Cytokeratin 18 抗体 (pSer33) (ABIN362392)
抗原
See all Cytokeratin 18 (KRT18) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer33
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特异性
- The antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer33 (A-A-S (p) -V-Y) derived from Human Keratin 18 (CK18) . Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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别名
- Keratin 18
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背景
- KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
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分子量
- 46 kDa
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NCBI登录号
- NP_000215
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UniProt
- P05783
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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