SAPK, JNK (pThr183) 抗体
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北京 101111
Quick Overview for SAPK, JNK (pThr183) 抗体 (ABIN362308)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr183
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特异性
- The antibody detects endogenous level of SAPK/JNK only when phosphorylated at threonine 183.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pThr183 (M-M-T (p) -P-Y) derived from Human SAPK, JNK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-SAPK, JNK (pThr183) antibody
适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (pTyr185) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (AA 166-215) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (AA 151-200), (pTyr185) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "MK-2206 induces cell cycle arrest and apoptosis in HepG2 cells and sensitizes TRAIL-mediated cell death." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 217-24, (2013) (PubMed).
: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." in: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).
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- SAPK, JNK
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别名
- SAPK/JNK
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背景
- Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
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分子量
- 54 kDa
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NCBI登录号
- NP_001128
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UniProt
- P53779
抗原
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