MEK1 + MEK2 抗体 (pSer217, pSer221)
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Quick Overview for MEK1 + MEK2 抗体 (pSer217, pSer221) (ABIN362236)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer217, pSer221
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特异性
- The antibody detects endogenous level of MEK1/MEK2 only when phosphorylated at serine 217/221.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer217, 221 (I-D-S (p) -M-A) derived from Human MEK1, MEK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-MEK1 + MEK2 (MEK1 + MEK2) (pSer217) antibody
MEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-MEK1 + MEK2 (MEK1 + MEK2) (pSer221) antibodyMEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-MEK1 + MEK2 (MEK1 + MEK2) (AA 193-242) antibodyMEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-MEK1 + MEK2 (MEK1 + MEK2) (AA 189-238) antibodyMEK1 + MEK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- MEK1 + MEK2
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别名
- MEK1/MEK2
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背景
- Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
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分子量
- 45 kDa
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UniProt
- Q02750
抗原
-
