VAV1 抗体 (pTyr174)
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Quick Overview for VAV1 抗体 (pTyr174) (ABIN362131)
抗原
See all VAV1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr174
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特异性
- The antibody detects endogenous level of Vav only when phosphorylated at tyrosine 174.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pTyr174 (E-I-Y (p) -E-D) derived from Human Vav. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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应用备注
- Western blotting: 1:500-1:1000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7966-74, (2009) (PubMed).
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- VAV1 (Vav 1 Oncogene (VAV1))
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别名
- Vav
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背景
- Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation
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分子量
- 95 kDa
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NCBI登录号
- NP_005419
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UniProt
- P15498
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling
抗原
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