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CDC25A 抗体 (pSer76)

CDC25A 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN362128
发货至: 中国
  • 抗原 See all CDC25A 抗体
    CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
    抗原表位
    • 34
    • 33
    • 21
    • 17
    • 15
    • 12
    • 9
    • 9
    • 8
    • 8
    • 7
    • 7
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    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer76
    适用
    • 235
    • 93
    • 77
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    人, 小鼠, 大鼠
    宿主
    • 244
    • 7
    克隆类型
    • 245
    • 6
    多克隆
    标记
    • 108
    • 21
    • 16
    • 15
    • 11
    • 11
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 3
    • 3
    • 3
    • 3
    • 3
    This CDC25A antibody is un-conjugated
    应用范围
    • 193
    • 124
    • 69
    • 40
    • 40
    • 32
    • 21
    • 17
    • 15
    • 8
    • 7
    • 6
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    特异性
    The antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
    纯化方法
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    免疫原
    Peptide sequence around phosphorylation site of pSer76 (M-G-S (p) -S-E) derived from Human cdc25A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    亚型
    IgG
    Top Product
    Discover our top product CDC25A Primary Antibody
  • 应用备注
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Piao, Lee, Xu, Gwak, Oh, Park, Ha: "CK1ε targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 3, pp. 531-7, (2011) (PubMed).

  • 抗原
    CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
    别名
    cdc25A (CDC25A 产品)
    别名
    cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody
    背景
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
    分子量
    70 kDa
    NCBI登录号
    NP_001780
    UniProt
    P30304
    途径
    Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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