ELK1 抗体 (pSer389)
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Quick Overview for ELK1 抗体 (pSer389) (ABIN361945)
抗原
See all ELK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer389
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特异性
- The antibody detects endogenous level of Elk1 only when phosphorylated at serine 389.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer389 (P-R-S (p) -P-A) derived from Human Elk-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))
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别名
- Elk1
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背景
- Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene
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分子量
- 62 kDa
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NCBI登录号
- NP_001107
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UniProt
- P19419
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
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