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ELK1 抗体 (pSer383)

This 兔 多克隆 antibody specifically detects ELK1 in WB 和 IHC. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN361880
发货至: 中国
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Quick Overview for ELK1 抗体 (pSer383) (ABIN361880)

抗原

See all ELK1 抗体
ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

适用

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人, 小鼠, 大鼠

宿主

  • 166
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克隆类型

  • 145
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多克隆

标记

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This ELK1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

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    pSer383

    特异性

    The antibody detects endogenous level of Elk-1 only when phosphorylated at serine 383.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed bychromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    Peptide sequence around phosphorylation site of pSer383 (T-L-S (p) -P-I) derived from Human Elk-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • 抗原

    ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

    别名

    Elk-1

    背景

    Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.

    分子量

    47 kDa

    NCBI登录号

    NP_001107

    UniProt

    P19419

    途径

    MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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