Fibrillarin 抗体
(2 references)Quick Overview for Fibrillarin 抗体 (ABIN361375)
抗原
See all Fibrillarin (FBL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for the ~34kDa Fibrillarin /Nop1p protein. It is expected that it will work on other mammal tissues.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein G purified culture supernatant
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免疫原
- Yeast nuclear preparations
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亚型
- IgG1
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anti-Fibrillarin (FBL) antibody
FBL 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) (AA 100-321) antibodyFBL 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) (AA 1-321) antibodyFBL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G9-E4 unconjugated
anti-Fibrillarin (FBL) (N-Term) antibodyFBL 适用: 人, 大鼠, 小鼠, 犬, Cow, 兔, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) (Internal Region) antibodyFBL 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) (AA 61-110) antibodyFBL 适用: 人, 大鼠, 小鼠, 犬, Cow, 猴, 豚鼠, 马 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) (N-Term) antibodyFBL 适用: 人, 大鼠, 小鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL 适用: 人, 小鼠, 猴 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Recommended Dilution: WB: 1:1,000 IF: 1:500 Quality Control: Western blots performed on each lot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- total IgG fraction + 10 mM Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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: "SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation." in: Nature structural & molecular biology, Vol. 25, Issue 11, pp. 1035-1046, (2019) (PubMed).
: "The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis." in: Oncotarget, Vol. 7, Issue 37, pp. 59519-59534, (2018) (PubMed).
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: "SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation." in: Nature structural & molecular biology, Vol. 25, Issue 11, pp. 1035-1046, (2019) (PubMed).
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- Fibrillarin (FBL)
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别名
- Fibrillarin (Nop1p)
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背景
- Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
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分子量
- '34 kDa
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基因ID
- 851548
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UniProt
- P15646
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途径
- Ribonucleoside Biosynthetic Process
抗原
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