desert Hedgehog 抗体 (N-Term)
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Quick Overview for desert Hedgehog 抗体 (N-Term) (ABIN360880)
抗原
See all desert Hedgehog (DHH) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- This antibody detects Mouse DHH N-terminal polypeptide, but not C-terminal one in Western blotting.
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纯化方法
- Protein A/G affinity chromatography
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免疫原
- Recombinant dhh N-terminal fragment
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亚型
- IgG2
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anti-Desert Hedgehog (DHH) (AA 241-383) antibody
DHH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) (AA 212-392) antibodyDHH 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) (AA 1-396) antibodyDHH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) antibodyDHH 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) (AA 210-310) antibodyDHH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F10 unconjugated
anti-Desert Hedgehog (DHH) (AA 1-396) antibodyDHH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) (AA 51-158) antibodyDHH 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) (AA 74-123) antibodyDHH 适用: 人, 小鼠, 大鼠, Cow, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) (Center) antibodyDHH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Desert Hedgehog (DHH) (AA 195-223) antibodyDHH 适用: 人 WB 宿主: 兔 Polyclonal RB39505 unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.
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缓冲液
- 0.2 μm filtered PBS solution, None
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- desert Hedgehog (DHH) (Desert Hedgehog (DHH))
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别名
- Desert Hedgehog / DHH
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物质类
- Species
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背景
- Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa Nterminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling. Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). Within the N-terminal peptide, mouse Dhh shares 97 % and 100 % amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74 % aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis. It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules. In humans, mutations of Dhh are associated with pure gonadal dysgenesis. Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum. Its upregulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis. Dhh is also expressed by Schwann cells and is upregulated following nerve injury. It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves.Synonyms: HHG-3
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基因ID
- 13363
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NCBI登录号
- NP_031883
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UniProt
- Q61488
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途径
- Hedgehog Signaling
抗原
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