BUB1B 抗体 (N-Term)
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北京 101111
Quick Overview for BUB1B 抗体 (N-Term) (ABIN360515)
抗原
See all BUB1B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody reacts to BUBR1 (BUB1B).
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BUB1B.
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亚型
- Ig Fraction
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应用备注
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ELISA: 1/1,000. Western Blot: 1/50 - 1/200. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
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别名
- BUB1B
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背景
- BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.Synonyms: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1
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基因ID
- 701, 9606
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UniProt
- O60566
抗原
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