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BUB1B 抗体 (N-Term)

This anti-BUB1B antibody is a 兔 多克隆 antibody detecting BUB1B in WB, IHC (p) 和 EIA. Suitable for 人.
产品编号 ABIN360515
发货至: 中国

Quick Overview for BUB1B 抗体 (N-Term) (ABIN360515)

抗原

See all BUB1B 抗体
BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

适用

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宿主

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克隆类型

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多克隆

标记

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This BUB1B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    N-Term

    特异性

    This antibody reacts to BUBR1 (BUB1B).

    纯化方法

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BUB1B.

    亚型

    Ig Fraction
  • 应用备注

    ELISA: 1/1,000. Western Blot: 1/50 - 1/200. Immunohistochemistry: 1/50 - 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS with 0.09 % (W/V) sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

    别名

    BUB1B

    背景

    BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.Synonyms: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1

    基因ID

    701, 9606

    UniProt

    O60566
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