GCK 抗体 (C-Term)
Quick Overview for GCK 抗体 (C-Term) (ABIN360274)
抗原
See all GCK 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
特异性
- This antibody reacts to GCK.
-
纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
-
免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GCK.
-
亚型
- Ig Fraction
-
-
anti-Glucokinase (Hexokinase 4) (GCK) (AA 1-465) antibody
GCK 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) antibodyGCK 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4G6 unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) (AA 101-200) antibodyGCK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) (AA 3-226) antibodyGCK 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) (AA 1-300) antibodyGCK 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) antibodyGCK 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) (AA 1-465) antibodyGCK 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) (AA 1-465) antibodyGCK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) (AA 194-410) antibodyGCK 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glucokinase (Hexokinase 4) (GCK) (N-Term) antibodyGCK 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.25 mg/mL
-
缓冲液
- PBS with 0.09 % (W/V) sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
-
- GCK (Glucokinase (Hexokinase 4) (GCK))
-
别名
- Glucokinase / Hexokinase-4
-
背景
- Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2), mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).Synonyms: GCK, HK4, HKIV, Hexokinase type IV, Hexokinase-D
-
基因ID
- 2645, 9606
-
UniProt
- P35557
-
途径
- MAPK Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
抗原
-
