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PGK1 抗体 (Middle Region)

This anti-PGK1 antibody is a 兔 多克隆 antibody detecting PGK1 in WB, IHC (p) 和 EIA. Suitable for 人 和 小鼠.
产品编号 ABIN359199
发货至: 中国

Quick Overview for PGK1 抗体 (Middle Region) (ABIN359199)

抗原

See all PGK1 抗体
PGK1 (Phosphoglycerate Kinase 1 (PGK1))

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This PGK1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    Middle Region

    特异性

    This antibody reacts to PGK1.

    纯化方法

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PGK1.

    亚型

    Ig Fraction
  • 应用备注

    ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry 1/50 - 1/100. Flow cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS with 0.09 % (W/V) sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    PGK1 (Phosphoglycerate Kinase 1 (PGK1))

    背景

    Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.Synonyms: Cell migration-inducing gene 10 protein, PGKA, PRP 2, Primer recognition protein 2

    基因ID

    5230, 9606

    UniProt

    P00558

    途径

    Cellular Glucan Metabolic Process
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