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CKB 抗体 (N-Term)

The 兔 多克隆 anti-CKB antibody (ABIN359133) specifically detects CKB in WB 和 EIA. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN359133
发货至: 中国
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Quick Overview for CKB 抗体 (N-Term) (ABIN359133)

抗原

See all CKB 抗体
CKB (Creatine Kinase, Brain (CKB))

适用

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人, 小鼠

宿主

  • 102
  • 69
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克隆类型

  • 103
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多克隆

标记

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This CKB antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 抗原表位

    • 34
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    N-Term

    特异性

    This antibody reacts to Creatine Kinase BB (CKB).

    纯化方法

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CKB.

    亚型

    Ig Fraction
  • 应用备注

    ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS with 0.09 % (W/V) sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    CKB (Creatine Kinase, Brain (CKB))

    别名

    CKBB

    背景

    Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKB isoform, present in many tissues but especially brain, is a cytoplasmic enzyme involved in energy homeostasis. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behaviour, mossy fibre field size and determination of seizure susceptibility. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.Synonyms: B-CK, CKB, Creatine kinase B chain, Creatine kinase B-type, Creatine kinase BB

    基因ID

    1152, 9606

    UniProt

    P12277
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