DNMT1 抗体 (pSer154)
(1 reference)Quick Overview for DNMT1 抗体 (pSer154) (ABIN358462)
抗原
See all DNMT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer154
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特异性
- This antibody detects Dnmt1 pSer154.
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纯化方法
- Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S154 of human Dnmt1.
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亚型
- Ig Fraction
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anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-270) antibody
DNMT1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibodyDNMT1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibodyKO Validated DNMT1 适用: 人 WB, IHC, IP, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1448-1594) antibodyDNMT1 适用: 人, 猴 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7C11A2 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 21-120) antibodyDNMT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-270) antibodyDNMT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1317-1616) antibodyDNMT1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2D10G8 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 881-914) antibodyDNMT1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB51461 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibodyDNMT1 适用: 人 WB, IHC, IP, ChIP 宿主: 小鼠 Monoclonal 60B1220-1 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1317-1616) antibodyDNMT1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D5G2 unconjugated
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应用备注
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ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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: "Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline." in: Human molecular genetics, Vol. 17, Issue 9, pp. 1306-17, (2008) (PubMed).
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: "Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline." in: Human molecular genetics, Vol. 17, Issue 9, pp. 1306-17, (2008) (PubMed).
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- DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
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别名
- DNMT1
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背景
- Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities.Synonyms: AIM, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, HsaI, MCMT
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分子量
- 183165 Da
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基因ID
- 1786, 9606
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UniProt
- P26358
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途径
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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