EIF4E 抗体 (pSer209)
Quick Overview for EIF4E 抗体 (pSer209) (ABIN358406)
抗原
See all EIF4E 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer209
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特异性
- This antibody detectsEIF4E pSer209.
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纯化方法
- Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S209 of human EIF4E.
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亚型
- Ig Fraction
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anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody
EIF4E 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-100) antibodyEIF4E 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D11 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 2B1 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 2-217) antibodyEIF4E 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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别名
- EIF4E
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背景
- EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.Synonyms: EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
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分子量
- 25097 Da
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基因ID
- 1977, 9606
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UniProt
- P06730
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途径
- BCR Signaling
抗原
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